Biochemistry Seminar Dr. John Aitchison CANCELLED
Dr. John Aitchison
Professor
Associate Director Institute for Systems Biology Seattle
Please Note: Dr. John Aitchison has cancelled his visit due to the weather in Chicago - his flights were cancelled.听 He will be scheduling a visit and seminar at another time.
鈥淪ystems Biology of organelle biogenesis鈥�
We exploit the model organism S. cerevisiae for developing systems biology approaches to understand peroxisomes and peroxisome biogenesis.听 Peroxisomes are ubiquitous intracellular organelles that perform a variety of metabolic functions, most notably fatty acid beta oxidation.听 Peroxisomes are remarkably dynamic and their biogenesis occurs in response to a variety of stimuli.听 In yeast, peroxisomes are dramatically induced by fatty acids. The laboratory focuses on understanding the network dynamics controlling this biogenesis response at multiple levels, including signaling, transcription, chromatin dynamics and organelle biogenesis, using functional genomics, proteomics and computational tools of molecular systems biology.
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Marelli, M., Smith, J. J., Jung, S., Yi, E., Nesvizhskii, A. I., Christmas, R. H., Saleem, R. A., Tam, Y. Y., Fagarasanu, A., Goodlett, D. R., Aebersold, R., Rachubinski, R. A. and Aitchison, J. D. (2004) Quantitative mass spectrometry reveals a role for the GTPase Rho1p in actin organization on the peroxisome membrane, J Cell Biol, 167: 1099-1112
Ramsey, S. A., Smith, J. J., Orrell, D., Marelli, M., Petersen, T. W., de Atauri, P., Bolouri, H. and Aitchison, J. D. (2006) Dual feedback loops in the GAL regulon suppress cellular heterogeneity in yeast, Nat Genet, 38: 1082-1087
Smith, J. J., Ramsey, S. A., Marelli, M., Marzolf, B., Hwang, D., Saleem, R. A., Rachubinski, R. A. and Aitchison, J. D. (2007) Transcriptional responses to fatty acid are coordinated by combinatorial control, Mol Syst Biol, 3: 115
Hood, L., Rowen, L., Galas, D. J. and Aitchison, J. D. (2008) Systems biology at the Institute for Systems Biology, Brief Funct Genomic Proteomic, 7: 239-248
Marelli, M., Nesvizhskii, A. I. and Aitchison, J. D. (2008) Identifying bona fide components of an organelle by isotope-coded labeling of subcellular fractions : an example in peroxisomes, Methods Mol Biol, 432: 357-371
Ratushny, A. V., Ramsey, S. A., Roda, O., Wan, Y., Smith, J. J. and Aitchison, J. D. (2008) Control of transcriptional variability by overlapping feed-forward regulatory motifs, Biophys J, 95: 3715-3723
Saleem, R. A., Knoblach, B., Mast, F. D., Smith, J. J., Boyle, J., Dobson, C. M., Long-O'Donnell, R., Rachubinski, R. A. and Aitchison, J. D. (2008) Genome-wide analysis of signaling networks regulating fatty acid-induced gene expression and organelle biogenesis, J Cell Biol, 181: 281-292
Smith, J. J. and Aitchison, J. D. (2009) Regulation of peroxisome dynamics, Curr Opin Cell Biol.听 Feb;21(1):119-26. Epub 2009 Jan 31.
Saleem RA, Rogers RS, Ratushny AV, Dilworth DJ, Shannon PT, Shteynberg D, Wan Y, Moritz RL, Nesvizhskii AI, Rachubinski RA, Aitchison JD. (2010) Integrated phosphoproteomic analysis of a signaling network governing nutrient response and peroxisome induction, Mol Cell Proteomics. 2010 Apr 15. [Epub ahead of print].