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UID:20251005T205547EDT-9941ckEx7d@132.216.98.100
DTSTAMP:20251006T005547Z
DESCRIPTION:Ovarian cancer is the fifth largest cause of cancer-related dea
 ths in women. The vast majority of ovarian cancers are epithelial ovarian 
 cancers and high-grade serous carcinoma (HGSC) is the most common and most
  lethal epithelial ovarian cancer. In the last 10 years\, it has been reco
 gnized that the cell of origin of most HGSCs is within the fallopian tube 
 epithelium\, instead of the ovarian surface epithelium. However\, the earl
 y events in disease progression still remain poorly defined\, because HGSC
  is usually diagnosed at advanced stages and lack of proper animal models 
 recapitulating human disease progression.\n\nMy group has recently develop
 ed a unique strategy for generating mouse ovarian cancer models\, which is
  a combination of in vivo fallopian tube electroporation\, Cre-mediated li
 neage tracing and CRISPR-mediated gene modifications. As proof-of-principl
 e\, we generated a highly metastatic HGSC model by targeting four tumor su
 ppressor genes. The female mice targeted these four genes generated ovaria
 n tumors within 5 months after electroporation and peritoneal metastasis w
 ithin 6 months. After 6 months\, ascites formation was observed in two thi
 rd of those females. Interestingly\, similar to human ovarian cancer patie
 nts\, we observed two metastatic patterns\, miliary and non-miliary.\n\nOu
 r unique strategy has several advantages over the current mouse cancer mod
 els\; 1) High flexibility permitting many gene combinations/modifications 
 and host genetic backgrounds to be tested\; 2) Control over the size and a
 rea of targeted cells (the low-frequency mosaic transfection pattern bette
 r recapitulates the sporadic nature of human tumorigenesis)\; 3) The abili
 ty to track genetically modified cells by fluorescent reporters\, permitti
 ng analysis of tumor initiation and early metastasis\; 4) Highly metastati
 c mouse models with immune competency.\n
DTSTART:20190927T150000Z
DTEND:20190927T160000Z
LOCATION:Room 1034\, McIntyre Medical Building\, CA\, QC\, Montreal\, H3G 1
 Y6\, 3655 promenade Sir William Osler
SUMMARY:Seminar - Modeling ovarian cancer in mice using in vivo electropora
 tion and CRISPR mediated genome editing
URL:/physiology/channels/event/seminar-modeling-ovaria
 n-cancer-mice-using-vivo-electroporation-and-crispr-mediated-genome-editin
 g-299670
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