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BEGIN:VEVENT
UID:20251218T125225EST-1148JpS3tD@132.216.98.100
DTSTAMP:20251218T175225Z
DESCRIPTION:PhD Oral Defence of Yi Zhang\, Food Science & Agricultural Engi
 neering\n\nA 78 kDa Transglutaminase (TGase) was purified from Antarctic k
 rill with specific activity of 53.5 U/mg. The enzyme was Ca2+ dependent an
 d was most active in the temperature range of 0–10o C. Addition of 0.1 U/m
 g into cold-set gelatin hydrogels improved its textural properties. Thermo
 stability and kinetics studies found the cold-active TGase was stable at 0
 –40o C but was readily inactivated above 60o C. The corresponding conforma
 tional states and structural changes were identified. The gene encoding kr
 ill TGase was obtained and the amino acids sequence was deduced. The conse
 rved domains and catalytic triad were predicted from homology modeling. A 
 gelatin high in α-chain was produced as substrate for TGase. Optimized con
 ditions of the alcalase-assisted process produced 32% of α-chain containin
 g 34% glycine and 16% imino acids. The amide absorption bands and thermal 
 decomposition of gelatin were characterized. Antioxidative peptides were a
 lso produced as substrates for TGase via proteolysis by alcalase versus tr
 ypsin. The identified peptide sequences were further subjected to predict 
 in vivo functions using molecular docking. The cold-active TGase was expre
 ssed in E. coli and the recovered enzymes were used for forming cold-set p
 eptides/gelatin hydrogels with improved antioxidant activity.\n\nEveryone 
 in the 9IÖÆ×÷³§Ãâ·Ñ community is welcome to attend a PhD Oral Defence. Please jo
 in us in celebrating the accomplishments of our PhD candidates.\n
DTSTART:20190807T171500Z
DTEND:20190807T171500Z
LOCATION:MS2-022\, Macdonald-Stewart Building\, CA\, QC\, St Anne de Bellev
 ue\, H9X 3V9\, 21111 Lakeshore Road
SUMMARY:PhD Oral Defence: A cold-active transglutaminase: purification\, ch
 aracterization\, expression & application in producing food hydrogels
URL:/macdonald/channels/event/phd-oral-defence-cold-ac
 tive-transglutaminase-purification-characterization-expression-application
 -298354
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