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9I制作厂免费.CA / Facility for Electron Microscopy Research / User Access

Training



Instrument Training and Operation

Training is a cornerstone of FEMR's mission. It empowers users to perform sample preparation and advanced imaging using state-of-the-art electron microscopes for a wide range of applications.

Training Structure

  1. Workshops and Specialized EM Courses

    • FEMR organizes workshops that unite experts and trainees to explore both traditional and cutting-edge applications of electron microscopy. For upcoming workshops, please refer to the Resources section.

    • Specialized electron microscopy courses are offered through undergraduate and graduate programs in 9I制作厂免费's Faculties of Medicine, Science, and Engineering. Visit the 9I制作厂免费 website for course calendars and details.

  2. Instrument and Equipment Training

    • Practical sessions focus on mastering instrument operation and acquiring high-quality EM images.

    • Introductory training is offered free of charge on Tuesday mornings each month (refer to Table 1 or the Training Request Form for dates and times).

    • Advanced one-on-one training is mandatory and tailored to individual requirements, often requiring multiple sessions.


Responsible Instrument Use

With over 250 researchers depending on the FEMR instruments, improper or negligent usage can significantly disrupt experiments. To safeguard operations:

  • Training sessions are standardized to ensure consistent and comprehensive guidance, minimizing instrument downtime.

  • Protocols for instrument use and sample preparation are available on the Protocols page, promoting safe and efficient operations.

By adhering to these practices, users help maintain the functionality and availability of FEMR's vital research infrastructure.

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Training Request and Sessions

To secure a spot in a training session, please complete and submit the Training Request Form. The form allows you to select the desired session and month for attendance.

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Table 1 - Schedule of General Training Sessions Offered Each Month

Week Instrument Instructor Building/Room
1 FEI Tecnai 12/Tecnai G2 Spirit TEM - operation, sample loading, etc. Jeannie Mui or S Kelly Sears Strathcona Anatomy & Dentistry Building, Room B/29
1 FEI Quanta 450 FE-ESEM and Leica EM ACE600 Sputter Coater David Liu or Angel Valdez W.H. Wong Building, Room 0360; Strathcona Anatomy & Dentistry Building, Room B/25
2 FEI Tecnai G2 F20 Cryo-STEM and Gatan Cryo-holder Model 626 Kaustuv Basu or Corbin Black

Strathcona Anatomy & Dentistry Building, Room B/31
2 Thermo Scientific Talos 200 kV TEM for Materials Science - Part 1 David Liu or Angel Valdez Strathcona Anatomy & Dentistry Building, Room B/30
3 FEI Tecani G2 F20 Cryo-STEM - Operation

Kaustuv Basu or Corbin Black

Strathcona Anatomy & Dentistry Building, Room B/31
3 Hitachi Ethos NX5000 FIB-SEM System Stephanie Besette or Weawkamol Leelapornpisit W.H. Wong Building, Room 1280
4 Thermo Scientific Talos 200 kV TEM for Materials Science - Part 2 David Liu or Angel Valdez Strathcona Anatomy & Dentistry Building, Room B/30
4 FEI Vitrobot Mk IV Kaustuv Basu or Corbin Black

Strathcona Anatomy & Dentistry Building, Room B/25

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User Levels

  • Level 1: Independent Users

  • Levels 2 & 3: Supervised Users

Users must adhere strictly to standardized protocols when operating microscopes. Operating outside these procedures without prior consultation with staff is prohibited.

Microscopes are regularly aligned by staff, and these alignments are stored as part of the standard operating procedures. Unsupervised alignment adjustments are not allowed. If a user misaligns a microscope, they will incur costs for realignment.

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User Level

Description
Level 1

A Level 1 user is a fully trained and independent operator, granted unrestricted access to the instruments and equipment for which they are certified. To qualify for Level 1 access, a user must demonstrate a minimum of 35 hours of experience within the previous six months.

To retain Level 1 access, users must maintain consistent engagement by completing either a minimum of five hours per month or 35 hours over the previous six months.
Level 2 A听Level 2听user can use instruments and equipment without supervision, provided staff are available to assist if necessary.听 This usually corresponds to weekdays between 9:00 am and 5:00 pm unless prior arrangements are made.
Level 3 After completing the general and advanced one-to-one training, a听Level 3听user can book instruments and equipment in consultation with and under staff supervision.听 This practice ensures that the user becomes more familiar with the operation of the instrument and enables staff to evaluate their progress.听 It typically requires two to three sessions.

Dedicated staff operate the FEI Titan Krios cryo-STEM, Helios Nanolab 660 DualBeam, and Hitachi Ethos NX5000 FIBSEM. Generally, training on these instruments is not provided. However, trainees are encouraged to familiarize themselves with their operation by conducting experiments alongside staff.

It is recommended that users aspiring to become more proficient or advanced in instrument operation discuss their level of interest during the Assessment Interview. This enables staff to tailor sessions to align with the user's goals. Note that learning to operate the Titan Krios is a comprehensive process, often requiring multiple sessions to observe and practice.

Refresher Training

If you do not regularly use the research infrastructure, you may need refresher training. This ensures you remain proficient in operating the equipment and stay informed about any recent changes to operating procedures. Refresher training is provided at no additional technical fees.

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