BEGIN:VCALENDAR
VERSION:2.0
PRODID:-//132.216.98.100//NONSGML kigkonsult.se iCalcreator 2.20.4//
BEGIN:VEVENT
UID:20251106T153935EST-46266JI3In@132.216.98.100
DTSTAMP:20251106T203935Z
DESCRIPTION:Dr. Michelle Scott\nPhD\nCollege of Life Sciences\nUniversity o
 f Dundee\, United Kingdom\n“Bioinformatic characterization of the nucleolu
 s”\n \nAlthough primarily known for its role in the biogenesis of\nribosom
 es\, the nucleolus is involved in numerous other fundamental\ncellular act
 ivities\, including cell cycle progression\,\nproliferation regulation and
  the response to numerous forms of\ncellular stress. Recent large scale pr
 oteomics projects have\nidentified thousands of nucleolar-associated prote
 ins\, many of\nwhich are dynamically localized to this compartment under d
 ifferent\nconditions\, revealing cyclical and conditional nucleolar\nassoc
 iation. To understand the numerous roles of the nucleolus in\nthe cell\, i
 t is necessary to define the complete dynamic nucleolar\nproteome and char
 acterize its resulting interaction network.\n \nTo address these questions
 \, we have investigated the most\nwell-known form of targeting to the nucl
 eolus and created an\nartificial neural network predictor of nucleolar loc
 alization\nsequences (NoLSs). We predict over 50% of proteins annotated as
 \nnucleolar in Uniprot to harbour a NoLS\, suggesting this is a widely\nus
 ed mechanism. We have also investigated the extent of nucleolar\nassociati
 on of human proteins by integrating gene and protein\ncharacteristics into
  a probabilistic framework\, thus extending the\ncoverage of the nucleolar
  proteome past the current experimental\ndetection limits. These predictio
 ns reveal that approximately 15%\nof human proteins are nucleolar-associat
 ed and that this group is\nsignificantly more evolutionarily conserved tha
 n non-nucleolar\nproteins. This characterization of the complete dynamic n
 ucleolar\nproteome has been used to filter and annotate our human predicte
 d\ninteractome (http://www.compbio.dundee.ac.uk/www-pips/index.jsp)\,\nwhi
 ch will be augmented to include the known nucleolar RNA content\nand its r
 elationship to the nucleolar proteome.\n \n \nScott MS\, Avolio F\, Ono M\
 , Lamond AI\, Barton GJ (2009)\, Human\nmiRNA precursors with box H/ACA sn
 oRNA features\, PLoS Comp Biol\,\n5(9):e1000507.\n \nMcDowall MD\, Scott M
 S\, Barton GJ\, (2009) PIPs: human\nprotein-protein interaction prediction
  database\, Nucleic Acids\nResearch\, 37(Database issue):D651-6.\n \nScott
  MS and Barton GJ (2007) Probabilistic prediction and\nranking of human pr
 otein-protein interactions\, BMC Bioinformatics\n8(1):239.\n \nScott M\, B
 oisvert FM\, Vieyra D\, Johnston RN\, Bazett-Jones DP\,\nRiabowol K\, (200
 1)\, UV induces nucleolar translocation of ING1\nthrough two distinct nucl
 eolar targeting sequences\, Nucleic Acids\nResearch\, 29(10): 2052-8.\n
DTSTART:20100511T140000Z
DTEND:20100511T140000Z
LOCATION:McIntyre Medical Building\, CA\, QC\, Montreal\, H3G 1Y6\, 3655 pr
 omenade Sir William Osler
SUMMARY:Biochemistry Seminar Dr. Michelle Scott
URL:/channels/event/biochemistry-seminar-dr-michelle-s
 cott-116935
END:VEVENT
END:VCALENDAR