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UID:20251009T142633EDT-1673J4EdbU@132.216.98.100
DTSTAMP:20251009T182633Z
DESCRIPTION:Dr. Mike Gray\n\nNewcastle University Biosciences Institute\, T
 he Medical School\, Newcastle upon Tyne\, UK\n\n \n\nTalk title: ‘‘CFTR in
 dependent pathways that regulate airway surface liquid pH’’\n\n \n\nAbstra
 ct: Airway surface liquid (ASL) pH homeostasis is disrupted in CF due to f
 aulty CFTR\, which compromises innate defence mechanisms\, predisposing to
  pulmonary failure. Therefore\, restoring ASL pH homeostasis is a potentia
 l therapy for people with CF\, particularly for those who cannot benefit f
 rom current highly effective modulator therapy (HEMT). However\, we lack a
  comprehensive understanding of the complex mechanisms underlying ASL pH r
 egulation\, particularly in the absence of a functional CFTR. Possible alt
 ernative targets that impact ASL pH include\; Na/H exchangers\, proton-ATP
 ase\, SLC26A ion transporters and TMEM16A\, a calcium-activated chloride c
 hannel. This talk will describe our recent work investigating the role of 
 TMEM16A and SLC26A family members (SLC26A4 and SLC26A9) in acid-base homeo
 stasis. To do this we employed CRISPR-Cas9 to knock-out the transporters i
 n primary airway epithelial basal cells lacking CFTR\, and then measured d
 ynamic changes in ASL pH under thin-film conditions in fully differentiate
 d airway cultures\, under normal and inflammatory conditions. Our results 
 show that TMEM16A and SLC26A4 both play complex roles in ASL pH homeostasi
 s\, but SLC26A9 does not\, in cells lacking CFTR. Overall\, this suggests 
 that both TMEM16A and SLC26A4 could be important alternative targets for A
 SL pH therapy in CF\, particularly for those people who do not produce any
  functional CFTR.\n\n \n\nThis seminar will be given online via Zoom. Deta
 ils in attached poster.\n
DTSTART:20230523T150000Z
DTEND:20230523T160000Z
SUMMARY:CFTRc seminar - Dr. Mike Gray
URL:/cftrc/channels/event/cftrc-seminar-dr-mike-gray-3
 48359
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